Hotspot

trnT-GGU-trnE-UUC

LSC · 25257-25894 · 638 bp

Back to Marathrum

Pi 0.0215

MarkerSeek score 61.90

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 638
variable_sites: 13
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	CGTGAAAGGGAGATGTCCTG	50.0	55.0	58.0	58.0	704-733 bp	1.0	84.9
trnT-GGU-trnE-UUC_p2	GAACCGATGACTTACGCCTT	AAGGGAGATGTCCTGAACCA	50.0	50.0	58.0	58.0	699-728 bp	1.0	84.8
trnT-GGU-trnE-UUC_p3	CCATGGCGTTACTCTACCAC	CGTGAAAGGGAGATGTCCTG	55.0	55.0	58.1	58.0	712 bp	0.5	59.4
trnT-GGU-trnE-UUC_p4	TGGCGTTACTCTACCACTGA	CGTGAAAGGGAGATGTCCTG	50.0	55.0	58.1	58.0	709 bp	0.5	59.2
trnT-GGU-trnE-UUC_p5	CCATGGCGTTACTCTACCAC	AAGGGAGATGTCCTGAACCA	55.0	50.0	58.1	58.0	707 bp	0.5	59.1