Hotspot

trnS-UGA-psbC

LSC · 21374-21656 · 283 bp

Back to Marathrum

Pi 0.0522

MarkerSeek score 76.70

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 283
variable_sites: 13
pi: 0.0522
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GGTTGATAGCTCCGGTCTTG	CTGCAGCCGGATTTGAAAAG	55.0	50.0	58.1	58.0	394-428 bp	1.0	86.0
trnS-UGA-psbC_p2	GATGGCCGAGTGGTTGATAG	CTGCAGCCGGATTTGAAAAG	55.0	50.0	58.1	58.0	405-439 bp	1.0	85.3
trnS-UGA-psbC_p3	GGGTTCGAATCCCTCTCTCT	CTGCAGCCGGATTTGAAAAG	55.0	50.0	58.2	58.0	343-377 bp	1.0	85.0
trnS-UGA-psbC_p4	GGTTGATAGCTCCGGTCTTG	CCCGTTCTTTCCATGACTCC	55.0	55.0	58.1	58.3	353-387 bp	1.0	84.3
trnS-UGA-psbC_p5	GATGGCCGAGTGGTTGATAG	CCCGTTCTTTCCATGACTCC	55.0	55.0	58.1	58.3	364-398 bp	1.0	83.6