Hotspot

psbZ-trnS-UGA

LSC · 20908-21283 · 376 bp

Back to Marathrum

Pi 0.0187
MarkerSeek score 57.30
Primer available yes
Label psbZ-trnS-UGA

Feature Metadata

label_name
psbZ-trnS-UGA
feature_type
igs
region
LSC
length_bp
376
variable_sites
7
pi
0.0187
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnS-UGA_p1 AGCAATTAACGCAAAAACAGC CAAGACCGGAGCTATCAACC 38.1 55.0 56.9 58.1 454 bp 1.0 63.9
psbZ-trnS-UGA_p2 GCAATTAACGCAAAAACAGCT CAAGACCGGAGCTATCAACC 38.1 55.0 56.9 58.1 453 bp 1.0 63.9
psbZ-trnS-UGA_p3 AGCAATTAACGCAAAAACAGC CTATCAACCACTCGGCCATC 38.1 55.0 56.9 58.1 443 bp 1.0 63.4
psbZ-trnS-UGA_p4 GCAATTAACGCAAAAACAGCT CTATCAACCACTCGGCCATC 38.1 55.0 56.9 58.1 442 bp 1.0 63.4
psbZ-trnS-UGA_p5 TGTTACTTGACCATCCATCAGG CAAGACCGGAGCTATCAACC 45.5 55.0 57.8 58.1 521 bp 1.0 63.0