Hotspot

ndhJ-trnF-GAA

LSC · 9582-10067 · 486 bp

Back to Marathrum

Pi 0.0289
MarkerSeek score 62.00
Primer available yes
Label ndhJ-trnF-GAA

Feature Metadata

label_name
ndhJ-trnF-GAA
feature_type
igs
region
LSC
length_bp
486
variable_sites
14
pi
0.0289
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-trnF-GAA_p1 AGAAAGGATTATATTGCACCCA CGGTAGAGCAGAGGACTGAA 36.4 55.0 55.2 58.5 593-594 bp 1.0 42.0
ndhJ-trnF-GAA_p2 AGAAAGGATTATATTGCACCCAA CGGTAGAGCAGAGGACTGAA 34.8 55.0 55.9 58.5 593-594 bp 1.0 42.0
ndhJ-trnF-GAA_p3 AGAAAGGATTATATTGCACCCA CAAATGGTCGGGATAGCTCA 36.4 50.0 55.2 57.4 615-616 bp 1.0 41.9
ndhJ-trnF-GAA_p4 AGAAAGGATTATATTGCACCCA ACAAATGGTCGGGATAGCTC 36.4 50.0 55.2 57.4 616-617 bp 1.0 41.9
ndhJ-trnF-GAA_p5 AGAAAGGATTATATTGCACCCAA CAAATGGTCGGGATAGCTCA 34.8 50.0 55.9 57.4 615-616 bp 1.0 41.9