Hotspot

rbcL-atpB

LSC · 3546-4550 · 1005 bp

Back to Marathrum

Pi 0.0185

MarkerSeek score 64.60

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 18
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	GCCTTGAATCCAACACTTGC	TTCAAGCGCAGAAACTCCAA	50.0	45.0	57.9	58.3	1100-1118 bp	1.0	80.4
rbcL-atpB_p2	GGTGACATAAGTCCCTCCCT	TTCAAGCGCAGAAACTCCAA	55.0	45.0	58.1	58.3	1064-1082 bp	1.0	80.2
rbcL-atpB_p3	GCCTTGAATCCAACACTTGC	GCAGAAACTCCAAGACCTGA	50.0	50.0	57.9	57.4	1093-1111 bp	1.0	78.7
rbcL-atpB_p4	GGTGACATAAGTCCCTCCCT	GCAGAAACTCCAAGACCTGA	55.0	50.0	58.1	57.4	1057-1075 bp	1.0	78.4
rbcL-atpB_p5	GCCTTGAATCCAACACTTGC	CCAAGACCTGAAGTAGTCGG	50.0	55.0	57.9	57.3	1084-1102 bp	1.0	77.8