Hotspot

psaC

SSC · 124341-124586 · 246 bp

Back to Maranthes

Pi 0.00271

MarkerSeek score 29.10

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TCAAGTGGTATTTTCTGGAAAGT	TCATTGTAGAAATTTACTAGATCGA	34.8	28.0	56.0	53.9	330-331 bp	1.0	40.5
psaC_p2	TCAAGTGGTATTTTCTGGAAAGT	TCATTGTAGAAATTTACTAGATCGAA	34.8	26.9	56.0	54.6	330-331 bp	1.0	40.5
psaC_p3	TCAAGTGGTATTTTCTGGAAAGT	TTCATTGTAGAAATTTACTAGATCGA	34.8	26.9	56.0	54.6	331-332 bp	1.0	40.5
psaC_p4	TTCAAGTGGTATTTTCTGGAAAGT	TCATTGTAGAAATTTACTAGATCGA	33.3	28.0	56.7	53.9	331-332 bp	1.0	40.5
psaC_p5	TCAAGTGGTATTTTCTGGAAAGT	CATTGTAGAAATTTACTAGATCGA	34.8	29.2	56.0	52.5	329-330 bp	1.0	40.5