Hotspot

rpl32-trnL-UAG

SSC · 119925-121228 · 1304 bp

Back to Maranthes

Pi 0.00716
MarkerSeek score 58.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1304
variable_sites
14
pi
0.00716
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACCGCGTTGAAAGCTTTTTC AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1429-1434 bp 1.0 79.5
rpl32-trnL-UAG_p2 GACCGCGTTGAAAGCTTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1430-1435 bp 1.0 79.5
rpl32-trnL-UAG_p3 ACCGCGTTGAAAGCTTTTTC CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1430-1435 bp 1.0 79.5
rpl32-trnL-UAG_p4 GACCGCGTTGAAAGCTTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1431-1436 bp 1.0 79.5
rpl32-trnL-UAG_p5 ACCGCGTTGAAAGCTTTTTC TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 1439-1444 bp 1.0 78.6