Hotspot

psbT-psbN

LSC · 78921-78999 · 79 bp

Back to Maranthes

Pi 0.0338

MarkerSeek score 61.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 79
variable_sites: 4
pi: 0.0338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	GGGATCCCTTCGAAGAACAC	50.0	55.0	57.6	58.0	148 bp	1.0	79.1
psbT-psbN_p2	TCGAGAACCGCCTAAAGTTC	AGGGATCCCTTCGAAGAACA	50.0	50.0	57.6	58.0	149 bp	1.0	78.9
psbT-psbN_p3	GAGAACCGCCTAAAGTTCCA	GGGATCCCTTCGAAGAACAC	50.0	55.0	57.5	58.0	146 bp	1.0	78.2
psbT-psbN_p4	GAGAACCGCCTAAAGTTCCA	AGGGATCCCTTCGAAGAACA	50.0	50.0	57.5	58.0	147 bp	1.0	78.1
psbT-psbN_p5	CGAGAACCGCCTAAAGTTCC	GGGATCCCTTCGAAGAACAC	55.0	55.0	58.6	58.0	147 bp	1.0	76.8