Hotspot

psaJ-rpl33

LSC · 71448-72007 · 560 bp

Back to Maranthes

Pi 0.0145

MarkerSeek score 72.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 560
variable_sites: 10
pi: 0.0145
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GCGTTGACATTCCCGTTTTT	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	584-614 bp	1.0	82.4
psaJ-rpl33_p2	ATCAATCGCTTTTTCCCGGA	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	605-635 bp	1.0	82.3
psaJ-rpl33_p3	TCAATCGCTTTTTCCCGGAT	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	604-634 bp	1.0	82.3
psaJ-rpl33_p4	TACTCTATGGTTCGGGGCTT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	645-675 bp	1.0	81.6
psaJ-rpl33_p5	TCAATCGCTTTTTCCCGGAT	TTTCGAACACAACCGGTACA	45.0	45.0	57.9	57.7	644-674 bp	1.0	81.4