Hotspot

petA-psbJ

LSC · 66855-67651 · 797 bp

Back to Maranthes

Pi 0.00167

MarkerSeek score 60.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 2
pi: 0.00167
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GACCCATTACGTGTTCAAGGA	GGGTCATATTCCGGATTGGG	47.6	55.0	58.0	57.8	950-955 bp	1.0	70.3
petA-psbJ_p2	CCCATTACGTGTTCAAGGACT	GGGTCATATTCCGGATTGGG	47.6	55.0	57.9	57.8	948-953 bp	1.0	70.3
petA-psbJ_p3	ACCCATTACGTGTTCAAGGAC	GGGTCATATTCCGGATTGGG	47.6	55.0	58.2	57.8	949-954 bp	1.0	68.9
petA-psbJ_p4	ACCCATTACGTGTTCAAGGA	GGGTCATATTCCGGATTGGG	45.0	55.0	56.8	57.8	949-954 bp	1.0	68.9
petA-psbJ_p5	GACCCATTACGTGTTCAAGGA	GGTCATATTCCGGATTGGGC	47.6	55.0	58.0	58.5	949-954 bp	1.0	68.2