Hotspot

rbcL-accD

LSC · 59857-60655 · 799 bp

Back to Maranthes

Pi 0.00418

MarkerSeek score 61.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 799
variable_sites: 5
pi: 0.00418
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTTGTGAAGTGTGGAAGGA	AACATCGAATTGAACCACCA	50.0	40.0	58.0	55.6	881-885 bp	1.0	61.0
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	AACATCGAATTGAACCACCA	55.0	40.0	58.0	55.6	914-918 bp	1.0	60.9
rbcL-accD_p3	AAATGGAGTCCTGAACTGGC	AACATCGAATTGAACCACCA	50.0	40.0	57.8	55.6	905-909 bp	1.0	59.6
rbcL-accD_p4	GCTTGTGAAGTGTGGAAGGA	ACATCGAATTGAACCACCAT	50.0	40.0	58.0	55.3	880-884 bp	1.0	59.1
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	ACATCGAATTGAACCACCAT	55.0	40.0	58.0	55.3	913-917 bp	1.0	59.1