Hotspot

trnG-UCC-trnfM-CAU

LSC · 39437-39632 · 196 bp

Back to Maranthes

Pi 0.0374

MarkerSeek score 79.60

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 196
variable_sites: 11
pi: 0.0374
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	217-281 bp	1.0	85.8
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	215-279 bp	1.0	84.7
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	238-302 bp	1.0	84.0
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	237-301 bp	1.0	84.0
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	CATAACGCGGAGTAGAGCAG	50.0	55.0	57.8	58.2	262-326 bp	1.0	83.6