Hotspot

trnT-GGU-psbD

LSC · 33779-35363 · 1585 bp

Back to Maranthes

Pi 0.00252

MarkerSeek score 64.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1585
variable_sites: 6
pi: 0.00252
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	TATGTTGTCCGCATAGTGGC	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1565-1785 bp	1.0	83.8
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1507-1727 bp	1.0	83.7
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1510-1730 bp	1.0	83.6
trnT-GGU-psbD_p4	TATGTTGTCCGCATAGTGGC	AAGCAATAGACCGGACCAAC	50.0	50.0	58.1	57.9	1595-1815 bp	1.0	83.0
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	AAGCAATAGACCGGACCAAC	55.0	50.0	58.1	57.9	1537-1757 bp	1.0	82.9