Hotspot

trnC-GCA-petN

LSC · 28938-29941 · 1004 bp

Back to Maranthes

Pi 0.00465

MarkerSeek score 58.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1004
variable_sites: 7
pi: 0.00465
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGTGTCGCCTGATTAACAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	1052 bp	1.0	77.9
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1088 bp	1.0	76.5
trnC-GCA-petN_p3	CTGCAAATCCTTTTTCCCCG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.6	58.2	1082 bp	1.0	76.4
trnC-GCA-petN_p4	GGGTGTCGCCTGATTAACAA	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.2	57.6	1113 bp	1.0	76.2
trnC-GCA-petN_p5	TCCTTTTTCCCCGGTTCAAA	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.5	58.2	1075 bp	1.0	75.7