Hotspot

rps2-rpoC2

LSC · 16836-17103 · 268 bp

Back to Maranthes

Pi 0.0106

MarkerSeek score 63.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 268
variable_sites: 4
pi: 0.0106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GTGCCATTCTTGGATCCCAT	CTTGTTCCACCACCGAGAAT	50.0	50.0	57.9	57.8	424-441 bp	1.0	81.8
rps2-rpoC2_p2	AATGAACTCCTGCTTCCAGC	CTTGTTCCACCACCGAGAAT	50.0	50.0	58.2	57.8	385-402 bp	1.0	81.5
rps2-rpoC2_p3	AAGGTGCCATTCTTGGATCC	CTTGTTCCACCACCGAGAAT	50.0	50.0	57.9	57.8	427-444 bp	1.0	81.2
rps2-rpoC2_p4	GTGCCATTCTTGGATCCCAT	CGAGGGGGAAAGGAGAGATA	50.0	55.0	57.9	57.6	445-462 bp	1.0	80.0
rps2-rpoC2_p5	AAGGTGCCATTCTTGGATCC	CGAGGGGGAAAGGAGAGATA	50.0	55.0	57.9	57.6	448-465 bp	1.0	79.4