Hotspot

ycf1

SSC · 128073-133712 · 5640 bp

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Pi 0.00603

MarkerSeek score 44.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5640
variable_sites: 34
pi: 0.00603
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGCATGGATCTTATTTGGTTCT	TGAAGCTCTAGACAAAGGGT	36.4	45.0	55.5	55.8	211 bp	1.0	41.9
ycf1_p2	AGCATGGATCTTATTTGGTTCT	TTGAAGCTCTAGACAAAGGGT	36.4	42.9	55.5	56.6	212 bp	1.0	41.9
ycf1_p3	CTGTTCAAATTCTCGGTAATCAGT	TGAAGCTCTAGACAAAGGGT	37.5	45.0	57.3	55.8	250 bp	1.0	41.6
ycf1_p4	TGTTCAAATTCTCGGTAATCAGT	TGAAGCTCTAGACAAAGGGT	34.8	45.0	56.2	55.8	249 bp	1.0	41.6
ycf1_p5	CTGTTCAAATTCTCGGTAATCAGT	TTGAAGCTCTAGACAAAGGGT	37.5	42.9	57.3	56.6	251 bp	1.0	41.6