Hotspot

matK

LSC · 2219-3751 · 1533 bp

Back to Manilkara

Pi 0.0111

MarkerSeek score 65.50

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1533
variable_sites: 17
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	AAGCCTTACTACATGCTGCT	CCTTGTTTTGACTGAATCGCA	45.0	42.9	56.9	57.4	1725-1731 bp	1.0	59.4
matK_p2	AAGCCTTACTACATGCTGCT	ACCTTGTTTTGACTGAATCGC	45.0	42.9	56.9	57.4	1726-1732 bp	1.0	59.3
matK_p3	AAGCCTTACTACATGCTGCT	TGTTTTGACTGAATCGCACT	45.0	40.0	56.9	55.9	1722-1728 bp	1.0	55.6
matK_p4	AAGCCTTACTACATGCTGCT	CTTGTTTTGACTGAATCGCACT	45.0	40.9	56.9	57.8	1724-1730 bp	1.0	54.6
matK_p5	AAGCCTTACTACATGCTGCT	TTGTTTTGACTGAATCGCACT	45.0	38.1	56.9	56.7	1723-1729 bp	1.0	53.7