Hotspot

infA

LSC · 83449-83682 · 234 bp

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Pi 0
MarkerSeek score 19.00
Primer available yes
Label infA

Feature Metadata

label_name
infA
feature_type
gene
region
LSC
length_bp
234
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
19.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA_p1 CAGCAAAAGGTGTCCCTCTT GAAGGAATCGGCGGAGAAAT 50.0 50.0 58.0 58.0 941-962 bp 1.0 80.1
infA_p2 CCCTGAATCCACAAGTACCG GAAGGAATCGGCGGAGAAAT 55.0 50.0 58.0 58.0 914-935 bp 1.0 80.1
infA_p3 CCTGAATCCACAAGTACCGG GAAGGAATCGGCGGAGAAAT 55.0 50.0 58.0 58.0 913-934 bp 1.0 80.1
infA_p4 GTCCCTCTTCTTGTACCCCT GAAGGAATCGGCGGAGAAAT 55.0 50.0 58.0 58.0 930-951 bp 1.0 79.9
infA_p5 CCCTCTTCTTGTACCCCTGA GAAGGAATCGGCGGAGAAAT 55.0 50.0 58.0 58.0 928-949 bp 1.0 79.9