Hotspot

trnL-UAG-ccsA

SSC · 118501-118594 · 94 bp

Back to Manilkara

Pi 0.0213

MarkerSeek score 63.20

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 94
variable_sites: 2
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	ACAATTGAAACAATCGAAAAGGA	50.0	30.4	57.9	55.6	217-218 bp	1.0	40.7
trnL-UAG-ccsA_p2	AAATTGGTAGACACGCTGCT	ACAATTGAAACAATCGAAAAGGA	45.0	30.4	57.8	55.6	224-225 bp	1.0	40.7
trnL-UAG-ccsA_p3	GGTAGACACGCTGCTCTTAG	ACAATTGAAACAATCGAAAAGGA	55.0	30.4	57.8	55.6	219-220 bp	1.0	40.7
trnL-UAG-ccsA_p4	GTAGACACGCTGCTCTTAGG	ACAATTGAAACAATCGAAAAGGA	55.0	30.4	57.8	55.6	218-219 bp	1.0	40.7
trnL-UAG-ccsA_p5	AATTGGTAGACACGCTGCTC	ACAATTGAAACAATCGAAAAGGA	50.0	30.4	58.3	55.6	223-224 bp	1.0	40.7