Hotspot

ycf15-trnL-CAA

IRb · 97400-97769 · 370 bp

Back to Manilkara

Pi 0.0162

MarkerSeek score 63.30

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRb
length_bp: 370
variable_sites: 6
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	AGGAGAAGGAAGGCTGGAAT	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	481 bp	1.0	80.8
ycf15-trnL-CAA_p2	AGGAGAAGGAAGGCTGGAAT	TGAAATGGTAGACACGCGAG	50.0	50.0	58.0	58.0	483 bp	1.0	80.7
ycf15-trnL-CAA_p3	GGAGAAGGAAGGCTGGAATG	AAATGGTAGACACGCGAGAC	55.0	50.0	58.0	58.0	480 bp	1.0	80.5
ycf15-trnL-CAA_p4	GGAGAAGGAAGGCTGGAATG	TGAAATGGTAGACACGCGAG	55.0	50.0	58.0	58.0	482 bp	1.0	80.5
ycf15-trnL-CAA_p5	AGGAGAAGGAAGGCTGGAAT	GCCTTGGTGGTGAAATGGTA	50.0	50.0	58.0	58.1	493 bp	1.0	80.1