Hotspot

rps11-rpl36

LSC · 83109-83219 · 111 bp

Back to Manilkara

Pi 0.0541

MarkerSeek score 81.80

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 111
variable_sites: 6
pi: 0.0541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTGAACCAATACGTCCATTC	ATTTGTTCCAACCCGAGACA	47.6	45.0	57.3	57.4	206-226 bp	1.0	63.8
rps11-rpl36_p2	CGTGAACCAATACGTCCATTC	TTTGTTCCAACCCGAGACAT	47.6	45.0	57.3	57.4	205-225 bp	1.0	63.8
rps11-rpl36_p3	CGTGAACCAATACGTCCATT	ATTTGTTCCAACCCGAGACA	45.0	45.0	56.2	57.4	206-226 bp	1.0	62.3
rps11-rpl36_p4	CGTGAACCAATACGTCCATT	TTTGTTCCAACCCGAGACAT	45.0	45.0	56.2	57.4	205-225 bp	1.0	62.3
rps11-rpl36_p5	CGTGAACCAATACGTCCATTC	AATTTGTTCCAACCCGAGACA	47.6	42.9	57.3	58.1	207-227 bp	1.0	60.5