Hotspot

petA-psbJ

LSC · 66206-67269 · 1064 bp

Back to Manilkara

Pi 0.0232

MarkerSeek score 74.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1064
variable_sites: 24
pi: 0.0232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCTGTTGTTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.8	57.6	1174-1176 bp	1.0	78.8
petA-psbJ_p2	AGCATCTGTTGTTTTGGCAC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1175-1177 bp	1.0	76.5
petA-psbJ_p3	GCATCTGTTGTTTTGGCACA	TGGTTCATATTCCGGATTGGG	45.0	47.6	57.8	57.8	1182-1184 bp	1.0	72.0
petA-psbJ_p4	GCATCTGTTGTTTTGGCACA	GGTTCATATTCCGGATTGGGT	45.0	47.6	57.8	57.8	1181-1183 bp	1.0	72.0
petA-psbJ_p5	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	56.8	57.6	1124-1126 bp	1.0	71.5