Hotspot

trnT-GGU-psbD

LSC · 33916-35508 · 1593 bp

Back to Manilkara

Pi 0.00648

MarkerSeek score 65.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1593
variable_sites: 10
pi: 0.00648
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1690-1735 bp	1.0	82.3
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1693-1738 bp	1.0	82.1
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1708-1753 bp	1.0	81.4
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1711-1756 bp	1.0	81.2
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1667-1712 bp	1.0	80.5