Hotspot

rps16-trnQ-UUG

LSC · 6505-7726 · 1222 bp

Back to Manilkara

Pi 0.014

MarkerSeek score 59.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1222
variable_sites: 15
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1302-1763 bp	1.0	84.1
rps16-trnQ-UUG_p2	TCCTGTCTTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1306-1767 bp	1.0	83.1
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1295-1756 bp	1.0	83.1
rps16-trnQ-UUG_p4	CCACAACGGATCCTGTCTTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1316-1777 bp	1.0	82.7
rps16-trnQ-UUG_p5	TGTCTTTCAAGTCGCACGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.4	58.1	1303-1764 bp	1.0	81.4