Hotspot

trnH-GUG-psbA

LSC · 95-630 · 536 bp

Back to Manilkara

Pi 0.0186

MarkerSeek score 68.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 536
variable_sites: 8
pi: 0.0186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TTAGTTATGGGCGAACGACG	CCCTCTAGACCTTGCTGCTA	50.0	55.0	58.1	58.2	559-665 bp	1.0	82.6
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	CCCTCTAGACCTTGCTGCTA	50.0	55.0	57.9	58.2	504-610 bp	1.0	82.3
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	CCCTCTAGACCTTGCTGCTA	50.0	55.0	58.2	58.2	505-611 bp	1.0	82.1
trnH-GUG-psbA_p4	TTAGTTATGGGCGAACGACG	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.1	58.3	610-716 bp	1.0	81.6
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	555-661 bp	1.0	81.3