Hotspot

ycf1

SSC · 130312-135882 · 5571 bp

Back to Manihot

Pi 0.0101

MarkerSeek score 40.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5571
variable_sites: 84
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTCAGTGAATAATTTGTCCAAGC	ACCTTTCAAAATCAAAAAGAAATCCA	37.5	26.9	58.0	57.0	170 bp	1.0	44.2
ycf1_p2	TGTCAGTGAATAATTTGTCCAAGC	ACAACCTTTCAAAATCAAAAAGA	37.5	26.1	58.0	53.8	173 bp	1.0	44.2
ycf1_p3	TGTCAGTGAATAATTTGTCCAAGC	ACAACCTTTCAAAATCAAAAAGAA	37.5	25.0	58.0	54.6	173 bp	1.0	44.2
ycf1_p4	TGTCAGTGAATAATTTGTCCAAGC	AACCTTTCAAAATCAAAAAGAAATCCA	37.5	25.9	58.0	57.5	171 bp	1.0	44.2
ycf1_p5	TGTCAGTGAATAATTTGTCCAAGC	AACAACCTTTCAAAATCAAAAAGA	37.5	25.0	58.0	54.6	174 bp	1.0	44.1