Hotspot

rpl33-rps18

LSC · 72159-72578 · 420 bp

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Pi 0.00635
MarkerSeek score 50.40
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
420
variable_sites
4
pi
0.00635
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGACACAATACGCCTAGTCG TCAATTCGATCCCCCGATTG 55.0 50.0 58.5 57.7 512-593 bp 1.0 78.2
rpl33-rps18_p2 GACACAATACGCCTAGTCGA TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 511-592 bp 1.0 75.8
rpl33-rps18_p3 TCGACACAATACGCCTAGTC TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 513-594 bp 1.0 75.8
rpl33-rps18_p4 CGACACAATACGCCTAGTCG ATTCGATCCCCCGATTGGAT 55.0 50.0 58.5 58.6 509-590 bp 1.0 75.2
rpl33-rps18_p5 AGAATCGACACAATACGCCT TCAATTCGATCCCCCGATTG 45.0 50.0 57.0 57.7 517-598 bp 1.0 74.8