Hotspot

rbcL-accD

LSC · 59497-60303 · 807 bp

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Pi 0

MarkerSeek score 51.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	AGATCGAATTGAACCACCGT	55.0	45.0	58.0	57.2	876-931 bp	1.0	77.6
rbcL-accD_p2	GCAGCAGTGGATACTTTGGA	AGATCGAATTGAACCACCGT	50.0	45.0	57.9	57.2	807-862 bp	1.0	77.0
rbcL-accD_p3	TTGAATTCGCAGCAGTGGAT	AGATCGAATTGAACCACCGT	45.0	45.0	58.2	57.2	815-870 bp	1.0	76.5
rbcL-accD_p4	TCGCAGCAGTGGATACTTTG	AGATCGAATTGAACCACCGT	50.0	45.0	58.3	57.2	809-864 bp	1.0	75.7
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	AGATCGAATTGAACCACCGT	55.0	45.0	57.7	57.2	862-917 bp	1.0	75.6