Hotspot

ndhC-trnV-UAC

LSC · 53178-54219 · 1042 bp

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Pi 0.0198

MarkerSeek score 63.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1042
variable_sites: 31
pi: 0.0198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCCTTTGCTAATCGGGGATA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	56.8	57.8	1075-1186 bp	1.0	74.6
ndhC-trnV-UAC_p2	CTCCGGCCCTTTGCTAATC	AGAAGGTCTACGGTTCGAGT	57.9	50.0	58.3	57.8	1081-1192 bp	1.0	74.2
ndhC-trnV-UAC_p3	TTCTCCGGCCCTTTGCTAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	59.4	57.8	1083-1194 bp	1.0	73.6
ndhC-trnV-UAC_p4	CCCTTTGCTAATCGGGGATAA	AGAAGGTCTACGGTTCGAGT	47.6	50.0	57.5	57.8	1075-1186 bp	1.0	72.6
ndhC-trnV-UAC_p5	CCCTTTGCTAATCGGGGATA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	56.8	57.5	1074-1185 bp	1.0	71.9