Hotspot

psbZ-trnG-GCC

LSC · 38557-39205 · 649 bp

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Pi 0.0144

MarkerSeek score 60.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 14
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	796-803 bp	1.0	82.7
psbZ-trnG-GCC_p2	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	792-799 bp	1.0	81.1
psbZ-trnG-GCC_p3	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	795-802 bp	1.0	80.0
psbZ-trnG-GCC_p4	TCTCCTGATGGTTGGTCGAG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.8	57.8	794-801 bp	1.0	76.3
psbZ-trnG-GCC_p5	CTTCTCCTGATGGTTGGTCG	GGGAGAAAAGAGGATAGGGGA	55.0	52.4	58.0	57.9	868-875 bp	1.0	75.2