Hotspot

trnD-GUC-trnY-GUA

LSC · 32180-32643 · 464 bp

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Pi 0.00287
MarkerSeek score 50.70
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
464
variable_sites
2
pi
0.00287
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 TTGGATTTTGATCCGTCGGG TGGCAATATGTCTACGCTGG 50.0 50.0 58.3 57.8 560-594 bp 1.0 80.3
trnD-GUC-trnY-GUA_p2 TTGGATTTTGATCCGTCGGG GGCAATATGTCTACGCTGGT 50.0 50.0 58.3 57.8 559-593 bp 1.0 80.3
trnD-GUC-trnY-GUA_p3 TTGGATTTTGATCCGTCGGG GGGGACGGACTGTAAATTCG 50.0 55.0 58.3 58.4 581-615 bp 1.0 79.4
trnD-GUC-trnY-GUA_p4 TTGGATTTTGATCCGTCGGG GGGACGGACTGTAAATTCGT 50.0 50.0 58.3 57.6 580-614 bp 1.0 79.2
trnD-GUC-trnY-GUA_p5 TTGGATTTTGATCCGTCGGG GATTCTTCCTGGGTCGATGC 50.0 55.0 58.3 58.4 614-648 bp 1.0 78.9