Hotspot

trnK-UUU

LSC · 4661-4697 · 37 bp

Back to Mangifera

Pi 0.027

MarkerSeek score 65.80

Primer available yes

Label trnK-UUU

Feature Metadata

label_name: trnK-UUU
feature_type: tRNA
region: LSC
length_bp: 37
variable_sites: 1
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	TCAGGATCAGTCGTGGTCTT	CCGATTGTACCTACAGATTCGA	50.0	45.5	58.1	57.4	155 bp	1.0	59.4
trnK-UUU_p2	TCAGGATCAGTCGTGGTCTT	TCCGATTGTACCTACAGATTCG	50.0	45.5	58.1	57.4	156 bp	1.0	59.4
trnK-UUU_p3	TTCAGGATCAGTCGTGGTCT	CCGATTGTACCTACAGATTCGA	50.0	45.5	58.1	57.4	156 bp	1.0	59.4
trnK-UUU_p4	TTCAGGATCAGTCGTGGTCT	TCCGATTGTACCTACAGATTCG	50.0	45.5	58.1	57.4	157 bp	1.0	59.4
trnK-UUU_p5	ACGACATGCTGGTTTTTCCA	CCGATTGTACCTACAGATTCGA	45.0	45.5	58.3	57.4	185 bp	1.0	57.4