Hotspot

rpl36-infA

LSC · 83180-83296 · 117 bp

Back to Mangifera

Pi 0.0631

MarkerSeek score 87.30

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 117
variable_sites: 7
pi: 0.0631
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 87.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	TCTCCTACGGATCAGTCGAC	ATGGTTCAGAAATGGAGGGC	55.0	50.0	58.0	57.9	234-237 bp	1.0	82.8
rpl36-infA_p2	CGAACCGAGGCCCTTATTTT	ATGGTTCAGAAATGGAGGGC	50.0	50.0	58.3	57.9	197-200 bp	1.0	82.0
rpl36-infA_p3	CTCTCCTACGGATCAGTCGA	ATGGTTCAGAAATGGAGGGC	55.0	50.0	57.8	57.9	235-238 bp	1.0	81.3
rpl36-infA_p4	TCTCCTACGGATCAGTCGAC	GTTCAGAAATGGAGGGCGTA	55.0	50.0	58.0	57.6	231-234 bp	1.0	80.9
rpl36-infA_p5	CTCCTACGGATCAGTCGACA	ATGGTTCAGAAATGGAGGGC	55.0	50.0	58.3	57.9	233-236 bp	1.0	80.6