Hotspot

rpoA-rps11

LSC · 82469-82533 · 65 bp

Back to Mangifera

Pi 0.0308

MarkerSeek score 65.70

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 2
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	ACTTCCACTGTAGTGTCCGA	AGAGATGCAGCATTACGAGC	50.0	50.0	58.0	58.1	228 bp	1.0	79.5
rpoA-rps11_p2	ACTTCCACTGTAGTGTCCGA	GAGATGCAGCATTACGAGCT	50.0	50.0	58.0	58.1	227 bp	1.0	79.5
rpoA-rps11_p3	TTCCACTGTAGTGTCCGAGT	AGAGATGCAGCATTACGAGC	50.0	50.0	58.0	58.1	226 bp	1.0	79.5
rpoA-rps11_p4	TTCCACTGTAGTGTCCGAGT	GAGATGCAGCATTACGAGCT	50.0	50.0	58.0	58.1	225 bp	1.0	79.5
rpoA-rps11_p5	CACTTCCACTGTAGTGTCCG	GAGATGCAGCATTACGAGCT	55.0	50.0	57.9	58.1	228 bp	1.0	79.1