Hotspot

psbE-petL

LSC · 67852-69248 · 1397 bp

Back to Mangifera

Pi 0.00648
MarkerSeek score 58.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1397
variable_sites
9
pi
0.00648
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA GCCGCTAGTAGAAAACCGAA 45.0 50.0 58.0 58.0 1550-1553 bp 1.0 80.8
psbE-petL_p2 TGACAAATAACCAACCCGCA AAGCCGCTAGTAGAAAACCG 45.0 50.0 58.0 58.0 1552-1555 bp 1.0 80.7
psbE-petL_p3 CAACCCGCAATGAATAGGGA GCCGCTAGTAGAAAACCGAA 50.0 50.0 57.9 58.0 1539-1542 bp 1.0 80.5
psbE-petL_p4 CAACCCGCAATGAATAGGGA AAGCCGCTAGTAGAAAACCG 50.0 50.0 57.9 58.0 1541-1544 bp 1.0 80.5
psbE-petL_p5 AGAACGTTCTCCTGTGCTTC GCCGCTAGTAGAAAACCGAA 50.0 50.0 57.8 58.0 1462-1465 bp 1.0 79.7