Hotspot

rbcL-accD

LSC · 59798-60411 · 614 bp

Back to Mangifera

Pi 0.00489

MarkerSeek score 61.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 614
variable_sites: 3
pi: 0.00489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	TGACGCCAACGAAATGATCA	55.0	45.0	58.0	58.2	816 bp	1.0	78.4
rbcL-accD_p2	AAATGGAGTCCTGAACTGGC	TGACGCCAACGAAATGATCA	50.0	45.0	57.8	58.2	807 bp	1.0	77.1
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	ACTGTGGCGATCCATTGATT	55.0	45.0	58.0	57.6	791 bp	1.0	76.6
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	CTGACGCCAACGAAATGATC	55.0	50.0	58.0	57.6	817 bp	1.0	76.4
rbcL-accD_p5	GGCTAGCAAATGGAGTCCTG	TGACGCCAACGAAATGATCA	55.0	45.0	58.3	58.2	814 bp	1.0	76.1