Hotspot

atpB-rbcL

LSC · 57603-58369 · 767 bp

Back to Mangifera

Pi 0.0118

MarkerSeek score 55.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 9
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AAACCCCAGGATCAGAAGGA	TCTTTAACGCCGGCTTTGAA	50.0	45.0	57.9	58.4	860-870 bp	1.0	77.3
atpB-rbcL_p2	AAACCCCAGGATCAGAAGGA	TAGTCTTTAACGCCGGCTTT	50.0	45.0	57.9	57.5	863-873 bp	1.0	76.8
atpB-rbcL_p3	AAACCCCAGGATCAGAAGGA	TTTAACGCCGGCTTTGAATC	50.0	45.0	57.9	57.4	858-868 bp	1.0	75.6
atpB-rbcL_p4	AAACCCCAGGATCAGAAGGA	CTTTAACGCCGGCTTTGAAT	50.0	45.0	57.9	57.4	859-869 bp	1.0	75.5
atpB-rbcL_p5	AAACCCCAGGATCAGAAGGA	ATAGTCTTTAACGCCGGCTT	50.0	45.0	57.9	57.3	864-874 bp	1.0	75.0