Hotspot

trnS-GGA-ycf3

LSC · 35783-36684 · 902 bp

Back to Mangifera

Pi 0.00555
MarkerSeek score 62.00
Primer available yes
Label trnS-GGA-ycf3

Feature Metadata

label_name
trnS-GGA-ycf3
feature_type
igs
region
LSC
length_bp
902
variable_sites
5
pi
0.00555
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-ycf3_p1 CCCTCGGTAAACAAAAGCCT CATTTATCCGCGATCTAGGCA 50.0 47.6 58.1 58.0 991-995 bp 1.0 71.4
trnS-GGA-ycf3_p2 ACGGAAAGAGAGGGATTCGA CATTTATCCGCGATCTAGGCA 50.0 47.6 58.2 58.0 1012-1016 bp 1.0 70.9
trnS-GGA-ycf3_p3 TTCCAATGCTACGCCTTGAA CATTTATCCGCGATCTAGGCA 45.0 47.6 57.8 58.0 962-966 bp 1.0 70.6
trnS-GGA-ycf3_p4 TGGGTGAAGGTACGGAAAGA CATTTATCCGCGATCTAGGCA 50.0 47.6 58.3 58.0 1023-1027 bp 1.0 69.9
trnS-GGA-ycf3_p5 GGGTGAAGGTACGGAAAGAG CATTTATCCGCGATCTAGGCA 55.0 47.6 57.6 58.0 1022-1026 bp 1.0 69.1