Hotspot

trnK-UUU-rps16

LSC · 4698-5791 · 1094 bp

Back to Mangifera

Pi 0.0106
MarkerSeek score 50.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1094
variable_sites
9
pi
0.0106
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.78
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCAGGATCAGTCGTGGTCTT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 1215-1452 bp 1.0 83.6
trnK-UUU-rps16_p2 TCAGGATCAGTCGTGGTCTT TCCCAATGAGCCGTTTATCG 50.0 50.0 58.1 58.1 1216-1453 bp 1.0 83.6
trnK-UUU-rps16_p3 TTCAGGATCAGTCGTGGTCT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 1216-1453 bp 1.0 83.6
trnK-UUU-rps16_p4 TTCAGGATCAGTCGTGGTCT TCCCAATGAGCCGTTTATCG 50.0 50.0 58.1 58.1 1217-1454 bp 1.0 83.6
trnK-UUU-rps16_p5 CAATTTCTTTTGGTCCCCGC CCCAATGAGCCGTTTATCGA 50.0 50.0 57.9 58.1 1332-1569 bp 1.0 83.6