Hotspot

ycf1

SSC · 124407-130115 · 5709 bp

Back to Mandragora

Pi 0.0109

MarkerSeek score 50.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5709
variable_sites: 92
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CAGTTCTTACGGCCTGTTCA	AACACGGGGAATCCAGAAAA	50.0	45.0	57.8	57.3	172-202 bp	1.0	80.9
ycf1_p2	ACGGCCTGTTCAAATCGATT	AACACGGGGAATCCAGAAAA	45.0	45.0	58.2	57.3	164-194 bp	1.0	80.9
ycf1_p3	ACGGCCTGTTCAAATCGATT	ACACGGGGAATCCAGAAAAT	45.0	45.0	58.2	57.1	163-193 bp	1.0	79.1
ycf1_p4	CAGTTCTTACGGCCTGTTCA	ACACGGGGAATCCAGAAAAT	50.0	45.0	57.8	57.1	171-201 bp	1.0	79.0
ycf1_p5	TCAGTTCTTACGGCCTGTTC	AACACGGGGAATCCAGAAAA	50.0	45.0	57.5	57.3	173-203 bp	1.0	78.5