Hotspot

ndhA

SSC · 120209-122452 · 2244 bp

Back to Mandragora

Pi 0.00713
MarkerSeek score 55.60
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2244
variable_sites
24
pi
0.00713
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGTTTGAGACAAGTCGTGAA TGGGAGAAGTTGATCGTTGA 40.0 45.0 55.2 56.5 2303-2304 bp 1.0 46.3
ndhA_p2 TTGTTTGAGACAAGTCGTGA TGGGAGAAGTTGATCGTTGA 40.0 45.0 55.2 56.5 2304-2305 bp 1.0 46.3
ndhA_p3 TTGTTTGAGACAAGTCGTGAA TGGGAGAAGTTGATCGTTGA 38.1 45.0 56.0 56.5 2304-2305 bp 1.0 44.7
ndhA_p4 TGTTTGAGACAAGTCGTGAATC TGGGAGAAGTTGATCGTTGA 40.9 45.0 56.9 56.5 2303-2304 bp 1.0 44.3
ndhA_p5 TGTTTGAGACAAGTCGTGAA TGGGAGAAGTTGATCGTTGAA 40.0 42.9 55.2 57.2 2303-2304 bp 1.0 44.3