Hotspot

rpl32-trnL-UAG

SSC · 113881-114693 · 813 bp

Back to Mandragora

Pi 0.0249
MarkerSeek score 61.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
813
variable_sites
30
pi
0.0249
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAATCGAACGGGGATTCCAG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.4 57.9 1540-1615 bp 1.0 79.1
rpl32-trnL-UAG_p2 GAATCGAACGGGGATTCCAG GTCCCGAAGAAATCCAACGA 55.0 50.0 58.4 57.9 1877-1952 bp 1.0 79.0
rpl32-trnL-UAG_p3 GAATCGAACGGGGATTCCAG CCCGAAGAAATCCAACGAGT 55.0 50.0 58.4 57.9 1875-1950 bp 1.0 78.9
rpl32-trnL-UAG_p4 GAATCGAACGGGGATTCCAG GAAGTAGCAAAGCCTTGGGT 55.0 50.0 58.4 58.1 2028-2103 bp 1.0 78.8
rpl32-trnL-UAG_p5 GAATCGAACGGGGATTCCAG TGAAGTAGCAAAGCCTTGGG 55.0 50.0 58.4 58.1 2029-2104 bp 1.0 78.8