Hotspot

rps8-rpl14

LSC · 81438-81611 · 174 bp

Back to Mandragora

Pi 0.00399

MarkerSeek score 57.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 1
pi: 0.00399
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCAATAGTGTCCCTACCCA	GCCCGGGAATTGAGAGAATT	50.0	50.0	57.7	57.9	251-258 bp	1.0	79.9
rps8-rpl14_p2	TTCGAACCACCCTTTTTCGA	GCCCGGGAATTGAGAGAATT	45.0	50.0	57.7	57.9	306-313 bp	1.0	79.0
rps8-rpl14_p3	AGCAATAGTGTCCCTACCCA	AATTTTTGGTGCAATCGCCC	50.0	45.0	57.7	58.2	267-274 bp	1.0	78.7
rps8-rpl14_p4	TTCGAACCACCCTTTTTCGA	AATTTTTGGTGCAATCGCCC	45.0	45.0	57.7	58.2	322-329 bp	1.0	77.8
rps8-rpl14_p5	TCGAACCACCCTTTTTCGAT	GCCCGGGAATTGAGAGAATT	45.0	50.0	57.4	57.9	305-312 bp	1.0	77.2