Hotspot

psbE-petL

LSC · 65662-66751 · 1090 bp

Back to Mandragora

Pi 0.0123

MarkerSeek score 48.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1090
variable_sites: 20
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.0	57.1	1293-1376 bp	1.0	73.9
psbE-petL_p2	TGACAAATAACCAACCCGCA	AGCCGCTAATAGAAAACCGA	45.0	45.0	58.0	57.0	1247-1330 bp	1.0	73.7
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.9	57.1	1282-1365 bp	1.0	73.6
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AGCCGCTAATAGAAAACCGA	50.0	45.0	57.9	57.0	1236-1319 bp	1.0	73.4
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	1200-1283 bp	1.0	72.5