Hotspot

ndhC-trnV-UAC

LSC · 50770-51795 · 1026 bp

Back to Mandragora

Pi 0.0102
MarkerSeek score 57.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1026
variable_sites
15
pi
0.0102
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTGCTAATCGGGGCTAACAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.0 57.8 1124-1166 bp 1.0 82.7
ndhC-trnV-UAC_p2 GCTAATCGGGGCTAACACTC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.1 57.8 1122-1164 bp 1.0 81.9
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1127-1169 bp 1.0 80.2
ndhC-trnV-UAC_p4 TTGCTAATCGGGGCTAACAC GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.0 57.5 1123-1165 bp 1.0 80.0
ndhC-trnV-UAC_p5 GGCTAACACTCCGGAAATGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 1113-1155 bp 1.0 79.9