Hotspot

rps16-trnQ-UUG

LSC · 5681-6346 · 666 bp

Back to Mandragora

Pi 0.0609

MarkerSeek score 93.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 60
pi: 0.0609
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 93.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	756-996 bp	1.0	84.9
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	739-979 bp	1.0	84.5
rps16-trnQ-UUG_p3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	757-997 bp	1.0	83.8
rps16-trnQ-UUG_p4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	754-994 bp	1.0	82.6
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	753-993 bp	1.0	82.6