primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAGCCGAGTACTCTACCGTT	TTGAAAAAGGGGCTCAACCT	20	20	50.0	45.0	58.2	57.5	20.3	0.0	0.638477	4029	4549	652-661	1.000000	76.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	TTGAAAAAGGGGCTCAACCT	20	20	50.0	45.0	58.2	57.5	20.3	0.0	0.638477	4029	4549	653-662	1.000000	76.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCAGTCGTGGTCTTCCAAAC	TTGAAAAAGGGGCTCAACCT	20	20	50.0	45.0	57.8	57.5	0.0	0.0	0.704356	4029	4549	738-747	1.000000	75.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CAGTCGTGGTCTTCCAAACT	TTGAAAAAGGGGCTCAACCT	20	20	50.0	45.0	57.8	57.5	0.0	0.0	0.710550	4029	4549	737-746	1.000000	75.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAAAGACACTAGCCGCACTT	TTGAAAAAGGGGCTCAACCT	20	20	45.0	45.0	57.7	57.5	0.0	0.0	0.726174	4029	4549	674-683	1.000000	75.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	5681	6346	756-996	1.000000	84.9
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5681	6346	739-979	1.000000	84.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	7.8	16.9	0.285346	5681	6346	757-997	1.000000	83.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	5681	6346	754-994	1.000000	82.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	5681	6346	753-993	1.000000	82.6
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	7641	8264	698-704	1.000000	52.7
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	7641	8264	747-753	1.000000	51.7
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	7641	8264	701-707	1.000000	51.6
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	7641	8264	700-706	1.000000	51.6
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	20	22	45.0	36.4	58.0	56.2	0.0	0.0	3.814520	7641	8264	699-705	1.000000	50.7
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	21	20	42.9	55.0	56.4	58.0	0.0	17.9	2.629866	29662	30709	1203-1224	1.000000	60.7
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	21	20	42.9	50.0	56.4	58.1	0.0	17.9	2.709475	29662	30709	1204-1225	1.000000	60.1
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	22	20	40.9	55.0	57.1	58.0	0.0	17.9	2.905239	29662	30709	1204-1225	1.000000	58.5
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGAAAAGCGGTAGGAACTAGA	CCCGTATTTCCCTGGGATTG	21	20	42.9	55.0	56.1	58.0	0.0	17.9	2.908951	29662	30709	1200-1221	1.000000	58.5
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AAGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	22	20	40.9	50.0	57.1	58.1	0.0	17.9	2.984848	29662	30709	1205-1226	1.000000	57.9
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	31109	31797	830-997	1.000000	79.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	31109	31797	809-976	1.000000	78.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	31109	31797	806-973	1.000000	78.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	31109	31797	792-959	1.000000	78.3
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.310083	31109	31797	827-994	1.000000	77.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	46731	47432	723-752	1.000000	83.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	46731	47432	731-760	1.000000	82.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	46731	47432	755-784	1.000000	82.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAGAATAGGGAAAAGCCGGC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.2	58.0	21.9	0.0	0.240656	46731	47432	779-808	1.000000	82.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AAGAATAGGGAAAAGCCGGC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.2	58.1	21.9	0.0	0.305465	46731	47432	787-816	1.000000	81.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TTGCTAATCGGGGCTAACAC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.229964	50770	51795	1124-1166	1.000000	82.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GCTAATCGGGGCTAACACTC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.330117	50770	51795	1122-1164	1.000000	81.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.539129	50770	51795	1127-1169	1.000000	80.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TTGCTAATCGGGGCTAACAC	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.0	57.5	0.0	0.0	0.573736	50770	51795	1123-1165	1.000000	80.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGCTAACACTCCGGAAATGA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.6	57.8	19.7	0.0	0.593873	50770	51795	1113-1155	1.000000	79.9
accD-psaI_p1	accD-psaI	1	AGTTATTTAAGCTCCACGCCT	AAGAAGCCATTGCAATTGCC	21	20	42.9	45.0	57.4	57.9	0.0	26.8	1.732679	59357	60121	871-885	1.000000	68.5
accD-psaI_p2	accD-psaI	2	AGTTATTTAAGCTCCACGCCT	TGCAATTGCCGGAAAGACTA	21	20	42.9	45.0	57.4	57.8	0.0	26.9	1.821806	59357	60121	861-875	1.000000	67.8
accD-psaI_p3	accD-psaI	3	AGTTATTTAAGCTCCACGCCT	CCGGAAAGACTAGGCCTACT	21	20	42.9	55.0	57.4	58.2	0.0	8.9	1.851201	59357	60121	853-867	1.000000	67.6
accD-psaI_p4	accD-psaI	4	AGTTATTTAAGCTCCACGCCT	AGACTAGGCCTACTAACGGC	21	20	42.9	55.0	57.4	58.3	0.0	8.9	1.939158	59357	60121	847-861	1.000000	66.9
accD-psaI_p5	accD-psaI	5	TTTAAGCTCCACGCCTTTTT	AAGAAGCCATTGCAATTGCC	20	20	40.0	45.0	56.2	57.9	0.0	26.8	1.952668	59357	60121	866-880	1.000000	66.8
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	GTCGTATCTTGCTCAGACCA	20	20	45.0	50.0	58.0	57.1	0.0	0.0	0.958793	65662	66751	1293-1376	1.000000	73.9
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	AGCCGCTAATAGAAAACCGA	20	20	45.0	45.0	58.0	57.0	0.0	0.0	0.991034	65662	66751	1247-1330	1.000000	73.7
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGACCA	20	20	50.0	50.0	57.9	57.1	6.4	0.0	0.992787	65662	66751	1282-1365	1.000000	73.6
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	AGCCGCTAATAGAAAACCGA	20	20	50.0	45.0	57.9	57.0	6.4	0.0	1.025029	65662	66751	1236-1319	1.000000	73.4
psbE-petL_p5	psbE-petL	5	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	20	20	55.0	50.0	58.2	57.1	0.9	0.0	1.139384	65662	66751	1200-1283	1.000000	72.5
rpl20-rps12_p1	rpl20-rps12	1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	69871	70672	958-959	1.000000	75.4
rpl20-rps12_p2	rpl20-rps12	2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	69871	70672	957-958	1.000000	75.4
rpl20-rps12_p3	rpl20-rps12	3	TTTTTGTTCGACGTCTCCGA	AAATGTCACGAAATCCCCCG	20	20	45.0	50.0	57.8	58.5	14.7	0.0	0.747365	69871	70672	918-919	1.000000	74.7
rpl20-rps12_p4	rpl20-rps12	4	TTCGACGTCTCCGAGCTATA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.8	58.5	14.7	0.0	0.770225	69871	70672	912-913	1.000000	74.5
rpl20-rps12_p5	rpl20-rps12	5	TTTTGTTCGACGTCTCCGAG	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	58.2	58.5	14.7	0.0	0.778131	69871	70672	917-918	1.000000	74.5
rps8-rpl14_p1	rps8-rpl14	1	AGCAATAGTGTCCCTACCCA	GCCCGGGAATTGAGAGAATT	20	20	50.0	50.0	57.7	57.9	0.0	29.3	0.322488	81438	81611	251-258	1.000000	79.9
rps8-rpl14_p2	rps8-rpl14	2	TTCGAACCACCCTTTTTCGA	GCCCGGGAATTGAGAGAATT	20	20	45.0	50.0	57.7	57.9	0.0	29.3	0.387412	81438	81611	306-313	1.000000	79.0
rps8-rpl14_p3	rps8-rpl14	3	AGCAATAGTGTCCCTACCCA	AATTTTTGGTGCAATCGCCC	20	20	50.0	45.0	57.7	58.2	0.0	0.0	0.456756	81438	81611	267-274	1.000000	78.7
rps8-rpl14_p4	rps8-rpl14	4	TTCGAACCACCCTTTTTCGA	AATTTTTGGTGCAATCGCCC	20	20	45.0	45.0	57.7	58.2	0.0	0.0	0.521680	81438	81611	322-329	1.000000	77.8
rps8-rpl14_p5	rps8-rpl14	5	TCGAACCACCCTTTTTCGAT	GCCCGGGAATTGAGAGAATT	20	20	45.0	50.0	57.4	57.9	1.7	29.3	0.612963	81438	81611	305-312	1.000000	77.2
ndhF-rpl32_p1	ndhF-rpl32	1	CGGCGAAAACTTTTTGTTGC	TGCTACCCAATATCCCTTCCT	20	21	45.0	47.6	57.7	57.9	0.0	0.0	1.420326	112939	113712	971-972	1.000000	71.2
ndhF-rpl32_p2	ndhF-rpl32	2	GCGAAAACTTTTTGTTGCCG	TGCTACCCAATATCCCTTCCT	20	21	45.0	47.6	57.7	57.9	3.8	0.0	1.420326	112939	113712	969-970	1.000000	71.2
ndhF-rpl32_p3	ndhF-rpl32	3	GCGAAAACTTTTTGTTGCCG	TGATGTCGAAGTGCGTTTTT	20	20	45.0	40.0	57.7	56.6	3.8	0.0	1.755085	112939	113712	918-919	1.000000	68.7
ndhF-rpl32_p4	ndhF-rpl32	4	GCGAAAACTTTTTGTTGCCG	TTGATGTCGAAGTGCGTTTT	20	20	45.0	40.0	57.7	56.6	3.8	0.0	1.755085	112939	113712	919-920	1.000000	68.7
ndhF-rpl32_p5	ndhF-rpl32	5	GCGAAAACTTTTTGTTGCCG	TTTGATGTCGAAGTGCGTTT	20	20	45.0	40.0	57.7	56.6	3.8	0.0	1.755085	112939	113712	920-921	1.000000	68.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GAATCGAACGGGGATTCCAG	TCCTAAGAGCAGCGTGTCTA	20	20	55.0	50.0	58.4	57.9	4.8	0.0	0.541224	113881	114693	1540-1615	1.000000	79.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GAATCGAACGGGGATTCCAG	GTCCCGAAGAAATCCAACGA	20	20	55.0	50.0	58.4	57.9	4.8	0.0	0.505896	113881	114693	1877-1952	1.000000	79.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GAATCGAACGGGGATTCCAG	CCCGAAGAAATCCAACGAGT	20	20	55.0	50.0	58.4	57.9	4.8	0.0	0.511709	113881	114693	1875-1950	1.000000	78.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GAATCGAACGGGGATTCCAG	GAAGTAGCAAAGCCTTGGGT	20	20	55.0	50.0	58.4	58.1	4.8	0.0	0.505889	113881	114693	2028-2103	1.000000	78.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GAATCGAACGGGGATTCCAG	TGAAGTAGCAAAGCCTTGGG	20	20	55.0	50.0	58.4	58.1	4.8	0.0	0.507247	113881	114693	2029-2104	1.000000	78.8
ndhA_p1	ndhA	1	TGTTTGAGACAAGTCGTGAA	TGGGAGAAGTTGATCGTTGA	20	20	40.0	45.0	55.2	56.5	0.0	0.0	4.321373	120209	122452	2303-2304	1.000000	46.3
ndhA_p2	ndhA	2	TTGTTTGAGACAAGTCGTGA	TGGGAGAAGTTGATCGTTGA	20	20	40.0	45.0	55.2	56.5	0.0	0.0	4.321373	120209	122452	2304-2305	1.000000	46.3
ndhA_p3	ndhA	3	TTGTTTGAGACAAGTCGTGAA	TGGGAGAAGTTGATCGTTGA	21	20	38.1	45.0	56.0	56.5	0.0	0.0	4.521934	120209	122452	2304-2305	1.000000	44.7
ndhA_p4	ndhA	4	TGTTTGAGACAAGTCGTGAATC	TGGGAGAAGTTGATCGTTGA	22	20	40.9	45.0	56.9	56.5	0.0	0.0	4.566807	120209	122452	2303-2304	1.000000	44.3
ndhA_p5	ndhA	5	TGTTTGAGACAAGTCGTGAA	TGGGAGAAGTTGATCGTTGAA	20	21	40.0	42.9	55.2	57.2	0.0	0.0	4.571175	120209	122452	2303-2304	1.000000	44.3
ycf1_p1	ycf1	1	CAGTTCTTACGGCCTGTTCA	AACACGGGGAATCCAGAAAA	20	20	50.0	45.0	57.8	57.3	0.0	0.1	0.831962	128888	129007	172-202	1.000000	80.9
ycf1_p2	ycf1	2	ACGGCCTGTTCAAATCGATT	AACACGGGGAATCCAGAAAA	20	20	45.0	45.0	58.2	57.3	4.4	0.1	0.846927	128888	129007	164-194	1.000000	80.9
ycf1_p3	ycf1	3	ACGGCCTGTTCAAATCGATT	ACACGGGGAATCCAGAAAAT	20	20	45.0	45.0	58.2	57.1	4.4	0.1	1.078087	128888	129007	163-193	1.000000	79.1
ycf1_p4	ycf1	4	CAGTTCTTACGGCCTGTTCA	ACACGGGGAATCCAGAAAAT	20	20	50.0	45.0	57.8	57.1	0.0	0.1	1.063121	128888	129007	171-201	1.000000	79.0
ycf1_p5	ycf1	5	TCAGTTCTTACGGCCTGTTC	AACACGGGGAATCCAGAAAA	20	20	50.0	45.0	57.5	57.3	0.0	0.1	1.118753	128888	129007	173-203	1.000000	78.5