Hotspot

trnI-GAU

SSC · 44505-44539 · 35 bp

Back to Mammillaria

Pi 0.0857

MarkerSeek score 75.80

Primer available yes

Label trnI-GAU

Feature Metadata

label_name: trnI-GAU
feature_type: tRNA
region: SSC
length_bp: 35
variable_sites: 3
pi: 0.0857
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU_p1	AATCGCGGCGTTCTTCTATT	GGGAAGGATGAATCGCTCTC	45.0	55.0	58.1	57.8	160 bp	1.0	79.7
trnI-GAU_p2	AATCGCGGCGTTCTTCTATT	GGAAGGATGAATCGCTCTCG	45.0	55.0	58.1	58.2	159 bp	1.0	79.2
trnI-GAU_p3	GTAATCGCGGCGTTCTTCTA	GGGAAGGATGAATCGCTCTC	50.0	55.0	58.2	57.8	162 bp	1.0	78.8
trnI-GAU_p4	AATCGCGGCGTTCTTCTATT	AACGGAGAGTTGTATGCTGC	45.0	50.0	58.1	58.3	184 bp	1.0	78.6
trnI-GAU_p5	AATCGCGGCGTTCTTCTATT	GGAGAGTTGTATGCTGCGTT	45.0	50.0	58.1	58.3	181 bp	1.0	78.6