Hotspot

trnV-GAC-rps12

SSC · 47590-48337 · 748 bp

Back to Mammillaria

Pi 0.00401

MarkerSeek score 61.10

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: SSC
length_bp: 748
variable_sites: 3
pi: 0.00401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	GTCAAGGTGACACTCTACCG	TGGCAACTTTACGTAAGGCA	55.0	45.0	57.7	57.7	829 bp	1.0	75.4
trnV-GAC-rps12_p2	GGTGGGGAGCAAGTCAAAAT	TGGCAACTTTACGTAAGGCA	50.0	45.0	58.4	57.7	906 bp	1.0	75.3
trnV-GAC-rps12_p3	CAGGCTCGAACTGATGACTT	TGGCAACTTTACGTAAGGCA	50.0	45.0	57.6	57.7	856 bp	1.0	75.2
trnV-GAC-rps12_p4	TGACACTCTACCGCTGAGTT	TGGCAACTTTACGTAAGGCA	50.0	45.0	58.4	57.7	822 bp	1.0	75.2
trnV-GAC-rps12_p5	TCAGGCTCGAACTGATGACT	TGGCAACTTTACGTAAGGCA	50.0	45.0	58.4	57.7	857 bp	1.0	74.6