Hotspot

rrn5-rrn4.5

SSC · 40375-41154 · 780 bp

Back to Mammillaria

Pi 0.261

MarkerSeek score 92.90

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: SSC
length_bp: 780
variable_sites: 171
pi: 0.261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 92.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	855-897 bp	1.0	91.3
rrn5-rrn4.5_p2	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	853-895 bp	1.0	91.3
rrn5-rrn4.5_p3	AGTATCGTCACCGCAGTAGA	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	853-895 bp	1.0	91.3
rrn5-rrn4.5_p4	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	851-893 bp	1.0	91.3
rrn5-rrn4.5_p5	AGTTCCTCTACGCCTAGGAC	CAGCTGAGGCATCCTAACAG	55.0	55.0	57.9	58.0	803-845 bp	1.0	91.2